首页|Single-cell transcriptomics reveals neural stem cell trans-differentiation and cell subpopulations in whole heart decellularized extracellular matrix
Single-cell transcriptomics reveals neural stem cell trans-differentiation and cell subpopulations in whole heart decellularized extracellular matrix
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The whole heart decellularized extracellular matrix(ECM)has become a promising scaffold material for cardiac tissue engineering.Our previous research has shown that the whole heart acellular matrix possesses the memory function regulating neural stem cells(NSCs)trans-differentiating to cardiac lin-eage cells.However,the cell subpopulations and phenotypes in the trans-differentiation of NSCs have not been clearly identified.Here,we performed single-cell RNA sequencing and identified 2,765 cells in the recellularized heart with NSCs revealing the cellular diversity of cardiac and neural lineage,con-firming NSCs were capable of trans-differentiating into the cardiac lineage while maintaining the origi-nal ability to differentiate into the neural lineage.Notably,the trans-differentiated heart-like cells have dual signatures of neuroectoderm and cardiac mesoderm.This study unveils an in-depth mechanism underlying the trans-differentiation of NSCs and provides a new opportunity and theoretical basis for cardiac regeneration.
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