The Estimation Model of Soluble Sugar Content in Stems and Leaves of Catalpa bungei C.A.Mey Based on Electrical Impedance Spectroscopy
The Catalpa bungei C.A.Mey was used to study soluble sugar content,membrane permeability and electrical impedance spectroscopy (EIS) of stems and leaves during frost hardening.The authors analysed the correlation between soluble sugar content and parameters of the EIS in stems and leaves.Using linear and non-linear regression analysis,Models of soluble sugar content of stems and leaves during frost hardening were built.The frost hardiness of Catalpa bungei C.A.Mey was assessed by EIS and electrolyte leakage (EL) methods.The results showed that:1) The levels of soluble sugar content of stems and leaves increased with the process of frost hardening.2) During frost hardening the reactance value in the EIS top arc decreased in stems.In contrast,the reactance value experienced an increment in EIS top arc in the initial stage,followed by a decline in leaves.3) The EIS parameter r was the best for assessing soluble sugar content of stems and the EIS parameter ψ for assessing soluble sugar content of leaves.The best models were r=-0.0177x2 + 0.889x + 8.5912,and ψ =0.0068x3-0.2023x2 + 1.2192x + 18.486 with the prediction accuracy of 91.62% and 81.06%,respectively.4) The frost hardiness values showed a great level of similarity when measured by r in stems and by ψ in leaves compared with the EL method.The mean absolute errors were 3.01 and 2.83,respectively.