首页|Probing lysosomal activity in vivo

Probing lysosomal activity in vivo

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Lysosomes are membrane-bound organelles for biomolecule degradation and recycling.They also serve as a nutrient sensing and signaling center to maintain cell and tissue homeostasis.Lysosomal properties alter in response to developmental or environmental cues,but these changes are hard to track in vivo.Employing C.elegans as a model system,we have developed assays to examine and quantify lysosome properties in vivo,including lysosome maturation,acidification and cleavage activity.These assays can be used to reveal alterations of lysosomal activity during C.elegans development and in stress conditions.

LysosomesC.elegansMaturationAcidificationCleavage activity

Xin Li、Yanan Sun、Xiaochen Wang

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National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China

College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Some strains were provided by the CGC,which is funded by NIH Office of Research Infrastructure ProgramsThis work was supported by the National Natural Science Foundation of ChinaThis work was supported by the National Natural Science Foundation of ChinaMinistry of Science and TechnologyStrategic Priority Research Program of the Chinese Academy of SciencesX.Li is supported by the National Natural Science Foundation of China

P40OD0104403163001917542032016YFA0500203XDB1900000031671401

2021

生物物理学报
中国生物物理学会 中国科学院生物物理研究所

生物物理学报

影响因子：0.355
ISSN：1000-6737
年,卷(期)：2021.7(1)
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