首页|A quantitative method to assess bacterial adhesion using recombinant bioluminescent Pseudomonas aeruginosa

A quantitative method to assess bacterial adhesion using recombinant bioluminescent Pseudomonas aeruginosa

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Bioluminescence technology has been widely used in the field of medical detection.The bioluminescent lux reporter system provides a non-invasive platform to monitor bacterial growth and expression in real time.This study aimed to establish a method for detecting bacterial adhesion on the surface of materials,including medical devices,by using recombinant bioluminescent Pseudomonas aeruginosa containing a lux reporter.By monitoring the growth and bioluminescent properties of the recombinant PAO1-lux strain,the optimal test conditions for bacterial adhesion detection in vitro were determined to be as follows: an initial inoculation density of 105 to 106 CFU/mL,M9 medium at a pH 6.2,an adhesion time of 6 h,and the collection of adherent bacteria by ultrasonic cleaning.The traditional CFU counting method and the bioluminescence method were compared,and the applicability of the new method was verified by testing the adhesion of bacteria on the surface of various materials.The validated bioluminescent strains could serve as strong candidates to be used as bacterial detection tools in applications such as bacterial adhesion evaluation as well as supplements and alternatives to traditional microbiological testing procedures.In addition,this method has the potential to enable the study of bacterial adhesion on the surface of inanimate objects and living tissues.With the development of this method and its wide applicability,it is expected to become a standard method for the detection of bacterial adhesion and the screening of anti-adhesion materials.

Bacterial adhesionPAO1-luxBioluminescence assayMedical materials

Lu Wang、Xinhua Qiao、Lei Gao、Chang Chen、Yi Wan

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Microbiology Institute of Shaanxi, Xi'an 710043, China

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

University of Chinese Academy of Sciences, Beijing 100049, China

Beijing Institute for Brain Disorders, Beijing 100069, China

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This work was supported by the Shaanxi Academy of Sciences ProjectThis work was supported by the Shaanxi Academy of Sciences ProjectFoundation of Science and Technology in Shaanxi ProvinceNational Natural Science Foundation of China

2020k-262018nk-012020TD-05031900893

2021

生物物理学报
中国生物物理学会 中国科学院生物物理研究所

生物物理学报

影响因子：0.355
ISSN：1000-6737
年,卷(期)：2021.7(1)
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