In silicon cloning and bioinformatic analysis of isopentenyl diphosphate isomerase genes in Taraxacum kok-saghyz
Two isopentenyl diphosphate isomerase genes(TkIDI1 and TkIDI2)were obtained successfully from Taraxacum kok-saghyz using in silicon cloning technique.Some characters of the IDI gene and encoded protein sequences were predicted and analyzed by the bioinformatics methods in the following aspects,such as phylogenetic tree,signal peptide,localization sites in cells,location of active sites,secondary and tertiary structure.Results showed that TkIDI1(980 bp)contains a complete ORF(696 bp)encoding 232 amino acid and was predicted to locate at endoplasmic reticulum or chloroplast;TkIDI2(1 038 bp)contains a complete ORF(843 bp)encoding 281 amino acid and was predicted to locate at mitochondrion or chloroplast;their truncated transcript productions were the cytoplasm located and presented PTS1 predicted as peroxisomal targeting.Protein structure prediction suggested that TkIDIs(TkIDI1 and TkIDI2)are highly homologous to other IDIs originate from various plants,and exhibit similar location of active sites and protein structure.
国家科技期刊平台
NSTL
万方数据
维普
