Analysis of codon bias of invertase genes in melon(Cucumis melo L.)
The sequence of invertase genes of melon was used for studying the codon bias of invertase genes to provide a basis for the improvement of flavor and quality of melon.The sequence of CDS of invertase genes of melon was analyzed by Codon W program,then the Relative synonymius codon usage (RSCU),Efective Number of Codon (ENC),and the content of GC,GC1s,GC2s,GC3s were analyzed by Mobyle program.The invertase genes of melon preferred to use condons ending with A or T.ATT,GTTand AGA were common preference used codon with RSCU value more than 1.However,GCG and CGG was low frequently used codon with RSCU value less than 1.It is showed that preferences of condon has a certain relationship with genetic relationship.Successful expression of target gene in exogenous gene expression system and enhancement of its expression level were achieved through increasing the dose of the target gene,optimizing the codon of target gene,improving culture conditions and other methods,which the optimization of codon of target gene has played a key role.
国家科技期刊平台
NSTL
万方数据
维普
