摘要
砷是一种致癌物,是心血管、外周血管疾病、神经疾病、糖尿病和各种癌症的致病因素.目的:利用 GO 数据库和KEGG数据库等生物信息学方法对GEO数据库数据中的差异表达基因进行评价.利用生物信息学分析软件对差异基因进行功能富集、功能注释分析和生存分析.利用Cytoscape上的蛋白-蛋白相互作用网络(Protein-protein interaction network,PPI)软件对 179 个差异基因进行筛选和分析.结果发现 126 个基因作用于蛋白靶点,其中有 10 个基因为关键基因分别为:PSMB3、HSP701、HSPE1、STIP1、HSPD1、HSP70、DNAJB1B、HSP90AA1.1、HSPA9H和TCP1.核心基因主要作用于内质网中的蛋白质加工通路.这可能会为砷对肝脏损伤的潜在生物标志物和生物学机制提供新的思路.
Abstract
Arsenic is a carcinogen and a causative agent of cardiovascular and peripheral vascular disease,neurological disorders,diabetes mellitus and various forms of cancer.Objective:Used Bioinformatics methods such as GO database and KEGG database to evaluate the differentially expressed genes in GEO database data.Methods:Functional enrichment,functional annotation analysis and survival analysis of the differential genes were performed by using bioinformatics analysis software.Results:Used protein-protein interaction network(PPI)software on Cytoscape to analyze 179 differential genes.A total of 126 genes were found to be acting on protein targets,and 10 of them were key genes,respectively:PSMB3,HSP701,HSPE1,STIP1,HSPD1,HSP70,DNAJB1B,HSP90AA1.1,HSP A9H,TCP1;Conclusion:The core genes mainly act on protein processing in endoplasmic reticulum pathway.It provides a new research direction for the potential biomarkers and biological mechanisms of arsenic induced liver injury.
基金项目
国家自然科学基金项目(81070128)
沈阳市高层次创新人才计划(RC170408)
沈阳医学院硕士研究生科研创新基金项目(Y20190501)
辽宁省重点研发计划项目(2018225013)
沈阳中青年高层次人才创新支持计划项目(RC180379)
国家科技期刊平台
NSTL
万方数据