Application of NGS-based chromatin sequencing in cancer research
All eukaryotic genomes are organized into a nucleoprotein complex called chromatin,which have a sophisticated and intricate three-dimensional structure.In addition to the basic DNA sequence,there also exist DNA modifications,DNA-protein interactions,DNA-DNA interactions and DNA-RNA interactions in chromatin,all of which,if altered,may play a vital role in cancer initiation and development.Through different chromatin sequencing techniques,researchers are able to parse these alterations and enhance our understanding of tumorigenesis,which could ultimately be applied to the cancer therapy.In this paper,we review some experimental principles and applications of common chromatin sequencing techniques.
国家科技期刊平台
NSTL
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