Construction of circRNA-miRNA-mRNA regulatory network in nodular goiter
To screen the differentially expressed circular RNA(circRNA)in nodular goiter based on bioinformatics analysis and reveal the role of circRNA-miRNA-mRNA regulatory network in nodular goiter.The microarray data of nodular goiter tissues are retrieved from GEO database,and the differentially expressed circRNA is screened by R software.Multiple bioinformatics databases are used to predict miRNAs and mRNA downstream,and target mRNA is enriched with GO and KEGG.The core genes are selected by STRING online database and Cytoscape software.Two circRNAs,42 miRNAs and 546 mRNA are identified.GO and KEGG enrichment analysis show that the target mRNA is mainly involved in cell growth and gene expression regulation.14 core genes(SP1,IGF1R,RPS6KB1,SMAD2,SMAD3,SMAD4,VEGFA,CCND1,CDK2,HSPA4,HIF1A,CREB1,NR3C1 and STAT5A)have been selected by the Cytoscape software.Finally,the circRNA-miRNA-mRNA regulatory network is constructed based on 2 circRNAs,11 miRNAs and 14 core mRNA.The abnormal expression of circRNA and related circRNA-miRNA-mRNA regulatory network in nodular goiter may become a new target for diagnosis and treatment of nodular goiter.
国家科技期刊平台
NSTL
万方数据
