Bioinformatics analysis of N6-methyladenosine modifications of differential expression gene in PBMCs from systemic lupus erythematosus
To analyze the N6-methyladenosine(m6A)modification profile in systemic lupus erythematosus(SLE)using bioinformatics methods and public databases.We establish an SLE m6A modification expression profile and analyze the potential roles of m6A-related differentially expressed genes(DEGs)in SLE using public data.DEGs are obtained from the ADEx database,and the m6A modification profile in SLE is analyzed using the m6A GEO dataset(GSE173312).Additionally,we perform GO/Pathway annotation analysis of m6A DEGs using DAVID.In SLE patients,the expression of the m6A writer components RBM15B and the eraser enzyme FTO are downregulated,while the expression of the reader IGFBP3 is upregulated.The SLE m6A modification profile include 181 genes,with 123 genes upregulated and 58 genes downregulated in expression.These genes are primarily involved in biological processes such as cell apoptosis and cell cycle pathways,type I interferon signaling pathway,DNA replication,and B cell MHC II molecule regulation.Abnormalities in PBMCs cell m6A modification are observed in SLE patients and may potentially contribute to the development and occurrence of the disease.
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