Development of a high intensity focused ultrasound device for bio-samples preparation
A miniaturized,low-cost high-intensity focused ultrasound device is developed for the problems of cross-contamination and uneven sample fragmentation in conventional ultrasound devices.This device generates ultrasonic waves through a concave spherical self-focusing piezoelectric ceramic piece,and creates a cavitation effect in the focusing area to achieve sample fragmentation.The feasibility of the device is demonstrated by physical simulation,then a driving circuit with adjustable power is designed and manufactured to generate 0～22.4 W acoustic power,and finally paraffin-embedded sample dewaxing experiments are performed to verify the validation of the device.The experimental results show that the dewaxing efficiency and safety of the high-intensity focused ultrasound device is significantly better than those of traditional chemical methods,and this device is comparable with commercial ultrasonic instruments.In summary,the high-intensity focused ultrasound device is expected to be applied in automated nucleic acid extraction and purification equipment and has a broad application prospect in the field of sample pre-processing.
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维普
