Effect of lncRNA HCG18 on biological behavior of nasopharyngeal carcinoma cells by targeting miR-140-3p/PD-L1 pathway
Objective To investigate the effect of lncRNA HCG18 on proliferation,migration,and invasion of nasopharyngeal carcinoma cells via microRNA-140-3p(miR-140-3p)/programmed death receptor ligand 1(PD-L1).Methods Human nasopha-ryngeal carcinoma CNE2 cells and normal nasal mucosal epithelial cells HNEpC cells were selected.CNE2 cells were divided into HCG18 group,pcDNA3.1 group,sh-HCG18 group,sh-NC group,miR-140-3p group,miR-NC group,miR-140-3p in-hibitor group,Scramble group,HCG18+miR-NC group,HCG18+miR-140-3p group,miR-140-3p inhibitor+sh-NC group and miR-140-3p inhibitor+sh-PD-L1 group.The CCK-8 was used to detect cell proliferation.Transwell test was used to detect cell invasion and migration.Western blot was used to detect PD-L1 protein expression,real-time fluorescence quantita-tive polymerase chain reaction(qRT-PCR)was used to detect levels of lncRNA HCG18,miR-140-3p and PD-L1 mRNA.The bioinformatics and dual luciferase report experiment were used to analyze targeting relationship of lncRNA HCG18,miR-140-3p and PD-L1.Results The levels of lncRNA HCG18,PD-L1 protein and mRNA in CNE2 cells were higher than those in HNEpC cells,while the level of miR-140-3p was lower than those in HNEpC cells(P＜0.05).After up-regulation of lncRNA HCG18 or down-regulation of miR-140-3p,the proliferation,migration and invasion of CNE2 cells were enhanced,and levels of PD-L1 protein and PD-L1 mRNA were increased(P＜0.05).After down-regulation of lncRNA HCG18 or up-regulation of miR-140-3p,the proliferation,migration and invasion of CNE2 cells were decreased,and levels of PD-L1 protein and PD-L1 mRNA were decreased(P＜0.05).MiR-140-3p showed targeted relationship with lncRNA HCG18 and PD-L1.Up-regulation of miR-140-3p expression was reversed the effect of up-regulation of lncRNA HCG18 on proliferation,migration and invasion of CNE2 cells,and knockdown of PD-Ll reversed the effect of down-regulation of miR-140-3p on proliferation,migration and invasion of CNE2 cells.Conclusion It is demonstrated that lncRNA HCG18 overexpression could up-regulate the level of PD-L1 via sponge forming miR-140-3p,promote the proliferation,migration,and invasion of CNE2 cells.
NETL
NSTL
万方数据
