Objective To investigate the effectiveness of konjac gulcomannan(KGM)polyacrylic acid(AA)dressing on diabetic wound healing.Methods KGM-AA dressing was fabricated by free radical polymerization,and water-absorption experi-ments were performed to examine water-absorbing and adhesive properties of KGM-AA.The micro-structure and morphol-ogy of KGM-AA were observed using scanning electron microscopy after KGM-AA lyophilization.The mouse fibroblasts L929 cells were co-cultured with KGM-AA for 24 hours,the cell proliferation and viability were estimated using live-dead staining kit and CCK-8 assay.The vascular endothelial cells were also used for KGM-AA co-culturing,and tube formation assay was performed.The vascular tube formation was observed at 2,4,6 and 8 hours after cell seeding.The diabetic model was established by intraperitoneal injection of streptozotocin(STZ)into mice,and the random blood glucose was greater than 11.1 mmol/L for two consecutive times.A circular wound with 1 cm in diameter were created on the back of mice,and the mice were randomly divided into 3 groups which were blank control group without treatment,and the other two groups which were treated with KGM and KGM-AA dressings respectively to observe wound healing effects.The hematoxylin-eosin(HE)staining was performed on wound skin after 7 and 14 days to observe wound healing,inflammation and hyperplasia.Results The KGM-AA exhibited excellent water-absorbing ability,and showed uniform porous structure with pore size of more than 10 μm after water absorption.After co-cultured with mouse L929,the cells successfully embedded within the KGM-AA,and embed-ded cells also showed excellent viability and proliferation ability,indicated that KGM-AA provided appropriate environment for cell attachment and growth.The results of tube formation experiment showed that the number of blood vessels in KGM-AA group was significantly higher than that in KGM group at about 6 hours after seeding,and the difference was statistically signifi-cant(P＜0.01).The difference was maintained until 10th hour after seeding.In vivo experiments showed that the wound skin of hyperglycemic mice treated with KGM-AA dressing was more complete than that of the control group at 7th day of treatment.Conclusion It is demonstrated that KGM-AA dressing shows excellent biocompatibility by providing porous protective film.More importantly,KGM-AA also exhibited pro-angiogenic property,which is beneficial for wound healing process,especially un-der hyperglycemic condition.KGM-AA can be considered a suitable dressing and cell delivery material.
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