Preparation and performance of multi-component fiber tendon anti-adhesion membrane with mechanical and struc-tural stability
Objective To study the feasibility of preparing tendon anti adhesion materials with excellent performance,with introducing of hydrophobic poly(caprolactone)(PCL)to improve the performance of chitosan(CS)/polyvinyl alcohol(PVA)blended films.Methods The raw materials(CS/PCL/PVA membranes,abbreviation as CPA membranes)were polyvinyl alcohol(PVA)with low protein adsorption,chitosan(CS)with fibroblast proliferation inhibition and hydrophobic polymer polycaprolactone(PCL)(CS∶PCL∶PVA=3∶5∶2,3∶4∶3,3∶3∶4,3∶2∶5).The composition of the anti-adhesion membrane was further characterized by Fourier transform infrared spectroscopy.The fiber membranes with different components were prepared by conjugate electrospinning technique.The effects of PVA and PCL content on morphology,mechanical and structural stability were investigated by scanning electron microscopy,universal tester and simulated body fluid pretreatment.Thermal properties,cytotoxicity and adhesion of the anti-adhesion membrane were further characterized by thermogravimetric analyzer and cell experiments.Results The infrared analysis results showed that there was no new characteristic peak in CS/PCUPVA membrane,indicating that preparation of fiber membrane did not generate any new functional groups or chemical bonds.The results of body fluid stability and microstructure test results showed that the introduction of PCL reduced fiber mean diameter of CPA membrane,and improved mechanical properties failure and crimp shrinkage of CS/PVA membrane in wet environment.Compared with CS/PVA membranes,the fracture strength and elongation of CPA membranes(CS∶PCL∶PVA=3∶5∶2)were increased by about 60％and 40％,respectively.The elastic modulus was comparable and consistent with the literature data.On the other hand,it improved the mechanical performance failure and solved curling and wrinkling problems of CPA films in wet environments.The results of thermogravimetric analysis results showed that the decomposition temperature of multi-component fiber membrane was 250 ℃-600 ℃,and actual application environment temperature was significantly lower than thermal decomposition temperature,which showed thermal stability,so anti-adhesion membrane would not lose structural performance due to temperature.The results of cell experiments showed that the CPA membrane(CS∶PCL∶PVA=3∶5∶2)had good cell compatibility and certain anti-cell adhesion in vitro.On 3rd day,the cell survival rates of CA and CPA fibrous membranes were(96.533±0.341)％and(101.877±11.001)％,respectively(P＞0.05).After 7 days of cultivation,the CPA optical density value was 0.223±0.002,significantly lower than that of CA(0.292±0.007).Conclusion The CPA membrane has enhanced mechanical strength and wet stability,which is more suitable for stress environment of tendon site.It has better anti-cell adhesion effect.The mass ratio of CS∶PCL∶PV A was 3∶5∶2,which is an optimal parameter for the anti-adhesion material.It has application potential in the field of anti-adhesion after tendon surgery.
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