Triptolide Inhibits LPS-induced Inflammatory Response in BV2 Microglia by Modulating the NLRP3 Pathway
Objective To investigate the mechanisation of triptolide TP inhibiting microglial inflammation based on the inflam-mosome(NLRP3)pathway.Methods BV2 microglia and lipopolysaccharide(LPS)were selected to stimulate microglia to promote cell inflammatory damage,and an in vitro model was established to observe the effect of TP on LPS-stimulated microglia.The BV2 microglia was divided into three experimental groups:control group,LPS-induced model group(1 μg/mL LPS),and TP group(1 nmol/L TP+1 μg/mL LPS).The control group was not treated,the other groups was treated by the drug for 24 h,then the morpholog-ical changes of the cells in the drug groups were observed by microscope and compared with the control group.Cell supernatants are determined by the Griess method for nitric oxide(NO)release;Immunofluorescence staining and Western blot assays were used to detect the levels of intercellular inflamamite protein 3(NLRP3),apoptosis-associated spot-like protein(ASC),cysteine aspartate pro-tease(caspase-1),and interleukin-18(IL-18).Results LPS-induced inflammatory activation of BV2 cells,which was amoebias-like,reduced cell activity and promoted the release of NO.After TP,it reduces NO levels,inhibits the activation of NLRP3 inflamma-somes,and the expression of the corresponding components NLRP3,ASC,caspase1 and IL-18 was dropped.Conclusion TP has an inhibitory effect on microglial inflammation,exerting its anti-inflammatory effect and inhibiting the activation and expression of NLRP3 inflammasomes.
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