Screening of Small Molecule Metabolite Markers in Urine of Male Lung Cancer Patients
Objective To screen and identify potential small molecule tumor markers from the urine samples of male lung cancer patients and healthy adults,providing a scientific basis for the early diagnosis and treatment of lung cancer.Methods Metabolomics analysis was performed on the urine samples of 13 male lung cancer patients and 49 healthy adults using gas chromatography-mass spectrometry(GC-MS).The compound information from the chromatograms was statistically analyzed to screen for small molecule metabolites with high match and detection rates.Results A total of 32 metabolites with a match percentage greater than 70%were identified,with six metabolites showing a detection rate greater than 50%.These metabolites include acetic acid,carbamic acid methyl ester,2-hydroxycineole/exo-2-hydroxycineole,2,3-pinanediol,5-methylhydantoin,and 4-methylphenol.In the urine of healthy adults,the detection rates of carbamic acid methyl ester and acetic acid were 89.79%and 38.78%,respectively.Conclusion The detection rates of these metabolites were significantly higher in lung cancer patients compared to the healthy population,with acetic acid and carbamic acid methyl ester both having a detection rate of 92.31%,and 2-hydroxycineole/exo-2-hydroxycineole and 2,3-pinanediol both having a detection rate of 76.92%.These findings lay the groundwork for subsequent research on tumor markers and suggest their potential application in the early diagnosis of lung cancer.
万方数据
维普
