Preparation of Fe3O4 Magnetic Carrier and Its Immobilization of Lipase
The Fe3O4 magnetic carrier was synthesized using a chemical co-precipitation method,and the lipase was immobi-lized on the Fe3O4 magnetic carrier through the adsorption method combined with the sol-gel method.Two methods were investigat-ed for immobilizing lipase:adsorbing lipase before or during the sol-gel process,and the activity recovery rates of both methods were measured.The experiments showed that the activity recovery rate of lipase immobilized via adsorption before the sol-gel pro-cess was comparatively higher.After optimizing the immobilization conditions,it was determined that under alipase concentration of 1.5 g/L,pH 8.5,temperature at 30℃,fixation time of 6 hours,the highest activity recovery rate of lipase achieved was 40.57%.After immobilization,the lipase exhibited good reusability and storage stability.The incorporation of magnetic carriers facilitates ef-ficient separation of lipase from the reaction system,thereby reducing operational costs.Furthermore,combining adsorption with sol-gel techniques enhanced the stability of lipase immobilization.
magnetic carriersol-gel methodadsorption methodimmobilized lipase
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