Fe3O4磁性载体的制备及其固定脂肪酶的研究
Preparation of Fe3O4 Magnetic Carrier and Its Immobilization of Lipase
徐然 1蔡佳慧1
作者信息
- 1. 山西大同大学化学与化工学院,山西 大同 037009
- 折叠
摘要
利用化学共沉淀法制备Fe3O4 磁性内核,并以其为载体采用吸附法结合溶胶-凝胶法固定脂肪酶.分别以内核包硅之前和包硅过程中加入脂肪酶两种方法固定脂肪酶,对两种方法得到的固定化脂肪酶测定酶活回收率.实验表明:先吸附脂肪酶后包硅所得酶活回收率较高.同时对固定条件进行优化,在给酶浓度1.5 g/L、pH 8.5、温度30℃、固定时长6h条件下进行包硅,所得固定酶酶活回收率最高为40.57%.且脂肪酶经过固定后具有了较好的重复使用性和储存稳定性.磁性载体的引入有利于脂肪酶从反应体系中分离,降低了使用成本.而吸附法与溶胶-凝胶法相结合,增加了脂肪酶的固定稳定性.
Abstract
The Fe3O4 magnetic carrier was synthesized using a chemical co-precipitation method,and the lipase was immobi-lized on the Fe3O4 magnetic carrier through the adsorption method combined with the sol-gel method.Two methods were investigat-ed for immobilizing lipase:adsorbing lipase before or during the sol-gel process,and the activity recovery rates of both methods were measured.The experiments showed that the activity recovery rate of lipase immobilized via adsorption before the sol-gel pro-cess was comparatively higher.After optimizing the immobilization conditions,it was determined that under alipase concentration of 1.5 g/L,pH 8.5,temperature at 30℃,fixation time of 6 hours,the highest activity recovery rate of lipase achieved was 40.57%.After immobilization,the lipase exhibited good reusability and storage stability.The incorporation of magnetic carriers facilitates ef-ficient separation of lipase from the reaction system,thereby reducing operational costs.Furthermore,combining adsorption with sol-gel techniques enhanced the stability of lipase immobilization.
关键词
磁性载体/溶胶-凝胶法/吸附法/固定化脂肪酶Key words
magnetic carrier/sol-gel method/adsorption method/immobilized lipase引用本文复制引用
出版年
2024
NETL
NSTL
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