以麦粒煮汁培养基作为双孢蘑菇液态发酵的基础培养基,采用响应面法对麦粒煮汁培养基进行了优化.双孢蘑菇在麦粒煮汁基础培养基中的菌丝体生物量和多糖产量分别为3.21 g/L和201.55 mg/L.在麦粒煮汁培养基中补充蛋白胨和麦芽糖后,其菌丝体生物量和多糖产量得到了显著提高(P＜0.05).响应面法优化培养基结果表明,最优麦粒煮汁培养基的配方为:1 000 mL麦粒煮汁,3.50 g蛋白胨,41.00 g麦芽糖,1.00 g KH2 PO4和0.50 g Mg-SO4.在最优麦粒煮汁培养基上,双孢蘑菇的菌丝体生物量和多糖产量分别达到12.33 g/L和579.93 mg/mL.
Optimization of Submerged Fermentation Medium for Agaricus bisporus by Response Surface Methodology
The aim of this work was to evaluate the potential of wheat extract as basal medium for the submerged fermentation of Agaricus bisporus and optimize the medium to obtain maximal mycelial biomass and intracellular polysaccharides (IPS) yield of this fungus.In the basal medium,the mycelial biomass and IPS yield of A.bisporus was respectively 3.21g/L and 201.55 mg/L.Supplementation of peptone and maltose to the basal medium caused a significant increase in either mycelial biomass or IPS yield as compared with control (P＜0.05).Through response surface methodology,the optimum wheat extract medium for the submerged fermentation of A.bisporus was the following:1 000 mL wheat extract,3.50 g peptone,41.00 g maltose,1.00 g KH2PO4 and0.50 g MgSO4.On the optimization of medium,the mycelial biomass and IPS yield of A.bisporus reached its maximum values of 12.33 g/L and 579.93 mg/L,respectively.
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