Study on the Mechanism of Reversing Drug Resistance of Leukemia Stem Cells by WeiCanQing Granule
This study explores the mechanism of action of WeiCanQing granule in reversing the resistance of leukemia stem cells to daunorubicin(DNR).Using the method of intervening CD34+CD38-KG1a leukemia stem cells with rabbit serum containing Wei-CanQing granule,CD34+CD38-KG1a cells sorted by immunomagnetic beads were divided into control group,DNR group,WeiCan-Qing granule group,and WeiCanQing granule+DNR group.Phosphatase and tensin homolog(PTEN)expression was silenced using siRNA method,and cell proliferation rate,cell cycle/apoptosis ratio,and Intracellular phosphatidylinositol kinase(PI3K)/phosphory-lated protein kinase B(p-AKT)/phosphorylated rapamycin target protein(p-mTOR)mRNA and protein levels were examined.The results showed that the combination of WeiCanQing granule containing serum and DNR significantly increased the proliferation inhi-bition rate,G2/M phase ratio,total apoptosis,and early apoptosis rate of CD34+CD38-KG1a cells.At the same time,the drug con-taining serum of WeiCanQing granule synergistically enhanced the expression of PTEN mRNA and protein,and reduces the expres-sion of PI3K,p-AKT,p-mTOR mRNA and protein.The above results suggest that WeiCanQing granule have the effect of reversing LSCs'resistance to DNR,and the mechanism of action may be related to negative regulation of PTEN on the PI3K/AKT/mTOR pathway.
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