铜-碳点复合物的制备及其抗菌活性研究
Preparation of Copper-Carbon Dots Complex and Its Antibacterial Activity
郭丽霞 1刘嘉欢 1周亮 2马颖钰 2张雪云 2刁海鹏 2刘文2
作者信息
- 1. 山西医科大学 药学院,山西 太原 030001
- 2. 山西医科大学 基础医学院,山西 太原 030001
- 折叠
摘要
铜绿假单胞菌(P.aeruginosa)是医院感染的主要病原体且耐药性强,故迫切需要开发新策略和发展高效药物分子来应对这个困境.本工作以柠檬酸和(2-甲基-5-硝基苯基)胍硝酸盐为原料,通过水热法合成了具有高效抗菌活性的蓝色荧光碳点(CDs).通过对CDs形貌、组分以及光学性能表征,显示CDs是粒径约3 nm且分散均一的纳米球,表面含有羧基和氨基等官能团,且在光刺激下可以产生活性氧.为了增强其抗菌活性,CDs进一步与Cu2+螯合得到复合物CDs@Cu.当浓度为30 µg/mL,在氙灯照射下相对于CDs对P.aeruginosa 85.4%的杀伤率,CDs@Cu的杀伤效率可达到99.9%.因此,该复合纳米粒子不仅可作为一种新型高效抗菌材料,而且对开发基于碳点的抗菌剂提供新思路和新方法.
Abstract
Pseudomonas aeruginosa(P.aeruginosa)is a major pathogen of hospital-acquired infections and has high drug resistance,so it is urgent to develop new strategies and efficient drug molecules to deal with this problem.In this paper,blue fluorescent carbon dots(CDs)with high antibacterial activity was synthesized by hydrothermal method using citric acid and(2-methyl-5-nitrophenyl)guanidine nitrate as raw materials.The morphology of CDs shows that they are the uniformly dispersed nanospheres with a particle size of about 3 nm.The composition and optical properties show that there are functional groups such as carboxyl and amino groups on the surface of CDs,and the CDs can generate reactive oxygen species under light stimulation.In order to improve antibacterial ac-tivity and the ability to promote wound healing,the CDs further chelated Cu2+to obtain composite CDs@Cu.The killing rate of CDs on P.aeruginosa under xenon lamp irradiation is 85.4%when the concentration was 30 µg/mL,and the killing efficiency of CDs@Cu can reach to 99.9%.Therefore,the composite nanoparticles can not only be used as a new and efficient antibacterial mate-rial,but also provided new ideas and methods for the development of antibacterial agents based on carbon dots.
关键词
铜绿假单胞菌/活性氧/铜离子/胍基Key words
Pseudomonas aeruginosa/reactive oxygen species/copper ion/guanidine group引用本文复制引用
基金项目
国家自然科学基金(82071969)
山西省自然科学基金(201901D111191)
山西省自然科学基金(20210302124341)
太原市迎泽区科技项目(2022)()
出版年
2024
国家科技期刊平台
NSTL
万方数据