Glutathione(GSH),cysteine(Cys),homocysteine(Hcy)and hydrogen sulfide(H2S)are important reactive sulfur species(RSS)in living organisms,which play important roles in physiological and pathological processes due to their antioxidant capacity.Herein,using N,N-diphenylamino as the electron donor group(D),thiophene as the π bridge,pyridine cation as the acceptor(A),and 2,4-dinitrophenyl(DNP)ether moiety as the recognition group,a ratiometric fluorescent probe,1-(4-(2,4-dinitrophenoxy)ben-zyl)-4-(2-(5-(diphenylamino)thiophen-2-yl)vinyl)pyridin-1-ium bromide(DTP)was designed and synthesized for detecting RSS.The interaction between DTP and RSS was studied by UV-Vis absorption spectrometry and fluorescence spectrometry.The results found that probe DTP itself showed a fluorescence emission at 642 nm;when RSS were added,the fluorescence peak blue shifted from 642 nm to 536 nm;and the fluorescence color of the solution changed from peach to yellow green under a 365 nm UV lamp.The simultaneous quantitative detection of RSS could be achieved through fluorescence intensity ratio(F536 nm/F642 nm).The high-reso-lution mass spectrometry(HR-MS)data confirmed that the reaction mechanism between DTP and RSS was mainly based on the cleavage of 2,4-dinitrophenylether by RSS.
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