首页|不同盐浓度处理下的紫球藻转录组测序分析

不同盐浓度处理下的紫球藻转录组测序分析

Transcriptomic Analysis of Porphyridium purpureum Under Different Salinity Treatments

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
转录组测序是挖掘调控微藻独特生物学功能及代谢通路关键基因的重要技术手段之一,但目前国内外对于不同盐浓度处理下的紫球藻(Porphyridium)转录组测序研究很少.为进一步挖掘不同浓度盐处理条件下紫球藻的功能基因,分析其参与的关键代谢通路,探究其在该藻类生长发育、生物活性物质合成及其产业化应用中的价值,本研究运用形态学、生理生化、分子生物及生物信息技术,分别对基础培养基条件(0.06 mol·L-1 NaCl)、中盐(1.0 mol·L-1 NaCl)和高盐(3.0 mol·L-1 NaCl)处理条件下的紫球藻(P.purpureum)进行特征及转录组测序分析.研究共获得65 790条单基因(Unigenes),其中CDS(Coding Sequence)序列49 653条,29.81%的Unigenes可被七大生物信息数据库注释,de novo组装了357条Contigs,其中N50长度5 467 bp,GC(Guanine and Cytosine)占比58.26%.差异表达基因(Differentially Expressed Genes,DEGs)可划分为3大簇,其中高盐处理组相关基因单独聚为一簇.以基础培养基处理组为对照,中、高盐浓度处理组共筛选获得上调表达DEGs 34 016条,下调表达DEGs 69 086条,共有DEGs 15 263条.不同比较组间共有DEGs的GO(Gene Ontology)富集程度最高的是细胞膜整体组分,KEGG(Kyoto Encyclopedia of Genes and Genomes)通路富集程度最高的是RNA转运.本研究所得紫球藻转录组测序数据及组装质量较高,筛选获得的DEGs及其功能信息可在该藻类高渗透压环境下生理机制研究、高品质微藻资源分子选育及模式微藻工业应用中提供重要的参考.
As an essential technology to screen the key genes with the unique biological functions and involved in the important meta-bolic pathways in microalgae,transcriptomic sequencing has been widely utilized in algae research.However,nowadays,a few stud-ies on the transcriptomic sequencing of Porphyridium under different salinity treatments have been reported around the world.In or-der to screen the functional genes of Porphyridium under different salt treatment conditions,analyze their related key metabolic path-ways,and explore their values in the growth and development,synthesis of bioactive substances,and industrial application,the mor-phological,physiological and biochemical,molecular biological and bioinformatic techniques have been used to feature analysis and transcriptomic sequencing of P.purpureum under the basic medium(0.06 mol·L-1 NaCl),moderate salt(1.0 mol·L-1 NaCl)and high salt(3.0 mol·L-1 NaCl)treatments,respectively.A total of 65 790 unigenes were obtained,including 49 653 coding domain sequenc-es(CDS).29.81%of them could be annotated among the seven bioinformatic databases.Totals of 357 contigs could be de novo as-sembled with a length of 5 467 bp for N50 and a GC ratio of 58.26%.Furthermore,differential expression genes(DEGs)could be di-vided into three major clusters via the cluster analyzing with the high salt treatment group clustered separately in this alga.Altogeth-er,34 016 up-regulated DEGs,69 086 down-regulated DEGs and 15 263 common DEGs were screened from the medium and high salinity treatment groups with the basic medium group as a control,respectively.The highest enriched Gene Ontology(GO)of DEGs among different comparative groups was the integral component of membrane.Meanwhile,the highest enriched Kyoto Ency-clopedia of Genes and Genomes(KEGG)pathway was RNA transport.To sum up,abundant transcriptomic sequencing data with high assembly quality of P.purpureum with different salt treatments have been obtained in this study.The DEGs and their functional annotations predicted in this alga will provide an important reference in for further researching on its high osmotic pressure adapt-ability and physiological mechanism,molecular breeding of high-quality microalgae resources and industrial application of the mod-el Porphyridium strains.

Porphyridium purpureumsalt tolerancetranscriptomic sequencingdifferential expression gene

高帆、宋韡、汤丽群

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山西大学 生命科学学院,山西 太原 030006

山西大学 教务处,山西 太原 030006

山西体育职业学院,山西 太原 030036

紫球藻 耐盐性 转录组测序 差异表达基因

山西省基础研究计划(自由探索类)教育部产学合作协同育人项目山西省高等学校教学改革创新项目山西省科技战略研究专项

202103021224009220504697124957J20220046202204031401051

2024

10.13451/j.sxu.ns.2024009

山西大学学报(自然科学版)
山西大学

山西大学学报(自然科学版)

CSTPCD北大核心
影响因子：0.287
ISSN：0253-2395
年,卷(期)：2024.47(4)
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