Effects of Resistance and Aerobic Intervention of Apoptosis-related Genes Expression in Tibialis Anterior Muscle of Mice
The present study is designed to determine the effects of resistance exercise and aerobic exercise on apoptotic signaling pathway in tibialis anterior muscles of different aged mice.Using young(3 months old)and older(6 months old)male senescence accelerated mouse prone/8(SAMP8)mice as the research animals,the resistance(ladder climbing)and aerobic(treadmill running)exercise models were established.The changes of key genes and their regulatory genes in endogenous apoptosis signaling pathway of tibialis anterior muscle in SAMP8 mice after 6-week exercise intervention were analyzed.Compared with the young group,the expression levels of Cyt C,Apaf-1 and Caspase-3 genes were significantly up-regulated in the old group,the Cyt C and Caspase-9 were up-regulated in young resistance training group,whereas Apaf-1,Caspase-9 and Caspase-3 showed significantly down-regula-tion in old resistance training group(P<0.05).After the aerobic exercise,the Apaf-1 and Caspase-9 mRNA expression increased sig-nificantly in young mice,and expression of Cyt C and Caspase-3 decreased significantly in old groups(P<0.05).For apoptosis-relat-ed regulatory factors,Bcl-2 gene expression in the young group was significantly up-regulated compared with the control group after resistance and aerobic exercise intervention,while Bcl-2,Bax and HSP70 gene expression in the old group were significantly down-regulated under the two forms of training intervention(P<0.05).Both resistance exercise and aerobic exercise can weaken the apop-totic signal in aging tibialis anterior muscles.Resistance exercise can reduce the expression of Caspase-3 in the tibialis anterior mus-cles of the aged group through the down-regulation of Apaf-1 and Caspase-9,whereas the down-regulation effect on Caspase-3 through aerobic training in the tibialis anterior muscle was mainly achieved by the decrease of Cyt C expression.Overall,the effects of the two forms of exercise interventions on the regulatory factors in the young groups tended to inhibit apoptosis,mainly through the up-regulation of Bcl-2.While the expression of apoptosis regulatory genes of the aged groups was down-regulated after exercise intervention,which may be explained by the relatively short intervention time.
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