本文采用液相色谱-质谱联用技术(liquid chromatograph-mass spectrometer,LC-MS),探究补充虾青素对急性大强度运动大鼠心肌代谢的影响.选取24只7周龄健康雄性SD(Sprague Dawley)大鼠,随机分为运动组(E组)和运动给药组(EM组).EM组灌服油溶虾青素,剂量为25 mg/(kg·d),E组以同样剂量灌胃大豆油;4周运动适应性喂养后,两组大鼠进行一次急性大强度跑台运动,然后立刻处死所有大鼠采集血液及心肌组织,血液用于检测心肌损伤标志物cTnI,心肌样品经处理后进行LC-MS检测得到对应代谢图谱,采用数据处理软件XCMS、SIM-CA-P、Excel、Origin等软件进行图像和数据处理与分析,找出差异代谢物并检索Met-PA及KEGG(Kyoto Encyclo-pedia of Genes and Genomes)数据库,分析筛选出靶标代谢标志物及靶标代谢通路.结果显示,两组大鼠心肌差异代谢物有15 种,参与了9 条代谢通路,其中虾青素对急性大强度运动机体作用的潜在靶标通路有3 条,分别是谷胱甘肽代谢、亚油酸代谢、半胱氨酸与蛋氨酸代谢通路.参与靶标代谢通路的标志物有6 种,分别是S-腺苷蛋氨酸、3-磷酸丝氨酸、1-硬脂酰基-2-油酰基卵磷脂(1-stearoyl-2-oleoylphosphatidylcholine,SOPC)、谷胱甘肽、亚油酸、5-L-谷氨酰-L-丙氨酸,它们均与降低机体氧化应激,减少心肌损伤相关.表明虾青素主要通过调节半胱氨酸和蛋氨酸代谢、亚油酸代谢、谷胱甘肽代谢通路起到调节氧化应激,预防心肌损伤,保护心脏的作用.
Metabolomic Study on the Effects of Astaxanthin on Myocardial Injury in Acute High-intensity Exercise Rats
To investigate the effects of astaxanthin supplementation on myocardial metabolism in rats with acute high-intensity exer-cise by liquid chromatography-mass spectrometry(LC-MS).Twenty four seven-week-old healthy male SD(Sprague Dawley)rats were randomly divided into two exercise groups(group E)and an exercise administration group(group EM).The EM group was giv-en oil-soluble astaxanthin(25 mg/(kg·d))and the E group was given the same dose soybean oil.The exercise methods of both groups were:one-time acute high-intensity treadmill exercise;intervention for 4 weeks;All rats were sacrificed immediately after the last acute high-intensity exercise,and the blood and myocardial tissue was collected.The cTnI in blood,a marker of myocardial injury was detected,after sample processing,the corresponding metabolic map was detected by LC-MS technology.For example,XCMS,SIMCA-P,Excel,Origin,and other software were used for image and data processing and analysis.The differential metabo-lites were identified,and the target metabolic markers and metabolic pathways were screened by meT-PA and KEGG databases.The results showed that there were 15 kinds of differential metabolites in the myocardium of rats in the two groups,which were involved in 9 metabolic pathways.The glutathione metabolism,linoleic acid metabolism,cysteine,and methionine metabolism pathways were identified as three potential target pathways of astaxanthin.There were six markers involved in the target metabolic pathway:S-adenosyl methionine,serine 3-phosphate,SOPC,glutathione,and linoleic acid 5-L-glutamyl-L-alanine,they are related to the reduc-tion of oxidative stress and myocardial injury.The results showed that astaxanthin can regulate oxidative stress,improve myocardial injury,and protect the heart by regulating the metabolism of cysteine and methionine,linoleic acid,and glutathione.
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