Analysis on Cloning and Expression Characteristics of VvWRKY70 Gene in Grape
Fresh-eating grapes need appropriate post-harvest preservation,and the gene reprogramming of fruit tissue caused by pre-servative treatment plays an important role in post-harvest preservation.Among the grape genes responsed to sulfur dioxide(SO2)preservative,we screened the VvWRKY70 gene(LOC100255013),which has the highest homology with AtWRKY70 of Arabidopsis.The promoter region of gene VvWRKY70 contained cis-acting elements in responses to abscisic acid,salicylic acid,methyl jasmo-nate and low temperature.The transgenic Arabidopsis expressing VvWRKY70 were obtained through Agrobacterium-mediated genet-ic transformation.The expression levels of VvWRKY70 in transgenic Arabidopsis plants were up-regulated under low temperature and Botrytis cinerea treatments,but down-regulated in SO2 exposure,which indicated that VvWRKY70 might participate in the re-sponses of plants to biotic and abiotic stresses.These results provide a basis for further study on the function and mechanism of grape gene VvWRKY70.
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