葡萄VvWRKY70基因的克隆及表达特性分析
Analysis on Cloning and Expression Characteristics of VvWRKY70 Gene in Grape
李莉娟 1刘婧 1郝丽宏 1仪慧兰1
作者信息
- 1. 山西大学 生命科学学院,山西 太原 030006
- 折叠
摘要
鲜食葡萄需要适当的采后保鲜措施,保鲜剂处理引发的果实组织基因重编程在采后保鲜中发挥了重要作用.在二氧化硫(SO2)保鲜剂诱导表达的葡萄基因中,我们筛选出与拟南芥AtWRKY70同源性最高的基因VvWRKY70,生物信息学分析发现VvWRKY70基因启动子区含有响应脱落酸、水杨酸、茉莉酸甲酯和低温的顺式作用元件;通过农杆菌介导的遗传转化获得了高表达VvWRKY70的拟南芥植株,该转基因植株在低温和灰霉菌胁迫时VvWRKY70上调表达,在SO2暴露时VvWRKY70下调表达,说明VvWRKY70参与了植物对生物和非生物胁迫的应答.这些结果为进一步研究葡萄基因VvWRKY70的功能及其作用机制提供了依据.
Abstract
Fresh-eating grapes need appropriate post-harvest preservation,and the gene reprogramming of fruit tissue caused by pre-servative treatment plays an important role in post-harvest preservation.Among the grape genes responsed to sulfur dioxide(SO2)preservative,we screened the VvWRKY70 gene(LOC100255013),which has the highest homology with AtWRKY70 of Arabidopsis.The promoter region of gene VvWRKY70 contained cis-acting elements in responses to abscisic acid,salicylic acid,methyl jasmo-nate and low temperature.The transgenic Arabidopsis expressing VvWRKY70 were obtained through Agrobacterium-mediated genet-ic transformation.The expression levels of VvWRKY70 in transgenic Arabidopsis plants were up-regulated under low temperature and Botrytis cinerea treatments,but down-regulated in SO2 exposure,which indicated that VvWRKY70 might participate in the re-sponses of plants to biotic and abiotic stresses.These results provide a basis for further study on the function and mechanism of grape gene VvWRKY70.
关键词
WRKY/葡萄/异源表达/转录调控Key words
WRKY/grape/heterologous expression/transcriptional regulation引用本文复制引用
基金项目
国家自然科学基金(31972132)
山西省专利推广实施资助专项(20210512)
小店区科技计划项目(202003S01)
出版年
2024
国家科技期刊平台
NSTL
万方数据