Biodiesel is one of the main substitute fuel to solve the problem of environment and energy. Salting-out extraction was used for recover)' of biodiesel, the results showed that the effect of sodium sulfate was the best. On the Hasis of single-factor experiment, second regression of central composite design, and establish to a mathematical models of regression equation, the optimal extraction conditions were as follows; sodium sulfate solution 5. 49 mol/L, extraction time 37 min, the predicted biodiesel rate was 97.99% ,the experimental value was 97.957% ,which was agreed with model predictions,the conditions obtained by regression analysis of central composite were applicable.