Optimization of ultrasonic-assisted extraction of total flavonoids from sugarcane leaves and its reducing power measurement
The total flavonoids from sugarcane leaves were extracted by ultrasonic-assisted.Using single factor experiment and orthogonal test experiment to determine the optimum extracting process of total flavonoids from sugarcane leaves and measured its reducing power.The results showed that the optimal ultrasonic-assisted extraction of flavonoids from sugarcane leaves are as follows:ultrasonic power 50 W,ultrasonic extraction temperature 70 ℃,liquid-to-material ratio 30∶1 mL/g,extraction time 25 min and ethanol volume fraction 80％.Under these conditions,the yield of total flavonoids was 18.37 mg/g and the total flavonoids from sugarcane leaves has some antioxidant activity.
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