Preparation of injectable temperature-sensitive hydrogel and in vitro evaluation
To prepare an injectable chitosan/β-sodium glycerophosphate temperature-sensitive hydrogel by ionic gelation method.The formulation was optimized by orthogonal experiment with gel time as indexes.The properties of temperature-sensitive hydrogel were studied by investigating the experiments of the effect of gel time of hydrogel with different concentration of NaCl and hydrogel' s stabilities and degradation.The results suggested 2％ chitosan concentration,β-sodium glycerophosphate concentration 40％,0.1 mol/mL HCl concentration,and the volume ratio of chitosan solution to sodium glycerophosphate solution of 5∶1 was chosen as the optimal formulation to prepare the hydrogel.With increasing of NaCl concentration solution added into hydrogel solution,the gel time was increased.The hydrogel was placed in 37 ℃ condition with 72 h became deteriorated,while the hydrogel still stable in-20 ℃.The degradation experiment of hydrogel indicated good compatible properties and had substantial application.
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维普
