It isolated a Gram positive Paenibacillus sp.strain from the soil at an altitude of 3 000 m in the Kalongla Mountains in Mutuo,Tibet.The entire genome of Paenibacillus sp.strain was sequenced by Illu-mina Hiseq and was annotated with Prokka software(v1.14.6).antiSMASH 7.0 and BAGEL4 were used to predict the secondary metabolites biosynthesis gene cluster in Paenibacillus sp.In order to validate the metabolic pathway,the total strain proteins were extracted by various extraction methods and performed nanoliter liquid chromatography combining with Mass Spectrometry.We could identify a total of 3 383 pro-teins by using software.Through homologous comparison,there were the N-terminal region of ATP depend-ent Cysteine protease B1 and ABC transporter in the lasso peptide biosynthesis gene cluster.It was com-plete by local blast comparison,and Paenibacillus sp.was likely to have the ability to synthesize lasso pep-tide.Through the above research,we analyed genomic and proteomic information of Paenibacillus sp.
NETL
NSTL
万方数据
