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外源酶强化厨余垃圾好氧堆肥降解及氮素转化

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中温淀粉酶、葡萄糖氧化酶及β-葡聚糖酶分别以60 mg/kg的比例加入厨余垃圾好氧堆肥堆体中,考察其对好氧堆肥过程堆肥周期、固氮效果、有机物降解的影响及其潜在机制。结果表明,尽管堆肥过程中三种外源酶添加没有强化高温期的峰值表现,但是中温淀粉酶和葡萄糖氧化酶可以使堆肥有机质降解率提高3。8%~4。1%,这可能归因于酶解预处理导致的酸积累。此外,中温淀粉酶和β-葡聚糖酶有效加速了堆肥的氨化过程,而葡萄糖氧化酶有利于硝态氮的累积,提升硝态氮含量6。0%,表明外源酶会产生酶的连锁反应,分别刺激β-葡萄糖苷酶和脱氢酶的活性。
Enhanced aerobic composting degradation and nitrogen conversion of kitchen waste by exogenous enzymes
The medium temperature amylase,glucose oxidase and β-gluconase,which were added to the pile at a ratio of 60 mg/kg.To investigate their effects and potential mechanisms on the composting cycle,nitrogen fixation effect,and organic matter degradation during the aerobic composting process.The results showed that although the addition of three exogenous enzymes did not enhance the peak performance dur-ing the high temperature period during the composting process,medium temperature amylase and glucose oxidase could significantly promote an increase in the organic matter degradation rate of compost by 3.8%~4.1%,which may be attributed to acid accumulation caused by enzymatic hydrolysis pretreatment.In addition,medium temperature amylase and β-gluconase effectively accelerated the ammonification process of compost,while glucose oxidase was beneficial for the accumulation of nitrate nitrogen,significantly increasing the nitrate nitrogen content by 6.0%.The results indicated that exogenous enzymes can pro-duce a chain reaction of enzymes,stimulating each other separately β-gluconase and dehydrogenase.

kitchen wasteaerobic compostingexogenous enzyme additionorganic matter degradationenzymatic activity

向远昆、何莹莹、张艺蝶、姚全福、谢丽

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长江水环境教育部重点实验室同济大学环境科学与工程学院,上海 200092

呼和浩特民族学院化学与环境学院,内蒙古呼和浩特 010051

厨余垃圾 好氧堆肥 外源酶添加 有机物降解 酶活性

国家重点研发计划国家自然科学基金

2019YFC190640222165010

2024

应用化工
陕西省石油化工研究设计院 陕西省化工学会

应用化工

CSTPCD北大核心
影响因子:0.411
ISSN:1671-3206
年,卷(期):2024.53(5)