Purification of the flavonoids from the flavoid extract of the Eucommia leaves and its antibacterial activity
The total flavonoids and four monomer compounds were purified from the Eucommia leaves fla-void extract,anti-inflammatory effects were preliminarily studied.The total flavonoid extract from Eucom-mia ulmoides leaves with a content of 65.19%,liquid quality analysis of its total flavonoids extract,solid-liquid extraction was used to further purification.The results showed that 95%ethanol extraction,extrac-tion material-to-liquid ratio of 1 g∶10 mL,and extraction frequency of 1 time were used,the extract with a total flavonoid content of Eucommia leaf was up to 80.35%;semi-prepared liquid phase was separated the eucommia flavonoid monomer compound;the anti-inflammatory activity was evaluated by measuring the inhibition effect of NO generation.Four monomer compounds were obtained by semi-prepared liquid phase separation,the four monomer compounds were quercetin-3-O-Sambunisglycoside,isoquercetin,rutin and viola vaeconin;they all had NO inhibition effect of NO generation,and the total flavonoid extract with 78.78%content was best,while monomer compound was viola vaeconin>rutin>quercetin-3-O-Sam-bunisglycoside>isoquercetin.
flavoid extract of Eucommia ulmoides leavespurification of the flavonoidsanti-inflammato-ry activity test
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