摘要
采用紫外分光光度计法,评估了一些杀菌剂对猕猴桃溃疡病菌的抑制活性,将两种活性较好的杀菌剂进行复配后,以纳米氢氧化镁为药物载体,研究了纳米氢氧化镁载体对复配杀菌剂的负载情况,并评估了纳米氢氧化镁对复配药物的抗猕猴桃溃疡病菌的影响,并对载体药物进行了田间应用效果研究.结果表明,测试的杀菌药剂都对猕猴桃溃疡病菌显示出一定的抑制活性,其中:复配药物-纳米氢氧化镁的抑菌活性最好,EC50 为 23.50 μg/mL,其次为复配药物,EC50为42.11 μg/mL.以纳米氢氧化镁作为药物载体,对药物的抗猕猴桃溃疡病菌有很好的协同作用,能提高药物的抑菌活性.另外,纳米氢氧化镁的含量对于载药量和包封率有显著影响.当氢氧化镁的含量从1mg增加到3.5 mg时,载药量增加了43%,包封率增加了 11%.此外,复配药物-纳米氢氧化镁载体剂对猕猴桃溃疡病有较好的田间防治效果,防效可达73.8%.
Abstract
The inhibitory activity of some fungicides against kiwifruit canker was evaluated by ultraviolet spectrophotometer. Mixing two active fungicides,nano-magnesium hydroxide was used as the drug carrier to study loading capacity to the mixed fungicide. The synergistic effect nano-magnesium hydroxide on the anti-kiwifruit canker bacteria of the mixed compound fungicide was evaluated. Besides,the field application effect of compounded drugs loaded with nano-Mg(OH)2 was studied. The results showed that the tested bactericidal agents all showed a certain inhibitory activity against kiwifruit canker. Among them,the compound drug- nano-Mg(OH)2 had the best antibacterial activity with an EC50 of 23. 50 μg/ mL,followed by the compound drug with an EC50 of 42. 11 μg/ mL. The use of nano-magnesium hydroxide as the drug carrier has a good synergistic effect on the drug against kiwifruit canker. It can significantly improve the antibacterial activity of the drug. And the content of nano-Mg(OH)2 has a significant effect on drug loading and encapsulation efficiency. When the magnesium hydroxide content was increased from 1 mg to 3. 5 mg,the drug loading increased by 43％ and the encapsulation efficiency increased by 11％. In addition, the compound drug- nano-Mg(OH)2 carrier agent has a good field control effect on kiwifruit canker, and the control effect can reach 73. 8％.
基金项目
贵州省科技计划项目(黔科合基础-ZK[2022]重点025)
贵州省高等学校教学内容和课程体系改革项目(2021214)
贵州省普通高等学校科技拔尖人才项目(黔教合KY字[2021]037)
维普
万方数据