The basic isolated and screened a novel strain HAUT-1 from activated sludge using pure culture technology,identified it by 16 S rRNA gene sequence analysis,and applied it in MFCs for power genera-tion testing and single-factor condition optimization.The results showed that the 16S rRNA sequence of strain HAUT-1 had the highest homology of 98%with Corynebacterium vitarumen(X84680),and thus the strain was named Corynebacterium vitarumen HAUT-1.The results of the single-factor test showed that the optimal power output conditions for this strain in MFCs were sodium acetate as the carbon source,glycine as the nitrogen source,incubation pH of 7,and incubation temperature of 25℃,under which the maxi-mum output voltage of strain HAUT-1 in MFCs could reach 635 mV;further analysis of polarization curves and power density curves showed that the open-circuit voltage of the MFCs system was 608 mV,and the output power density reached the maximum of 53.88 mW/cm2 when the current density was 24 mA/cm2.The results showed that the screened strain HAUT-1 in this study has a stable and highly ef-ficient power output capacity,which is of great significance for the development of electrogenic strain re-sources to further enhance the efficiency of MFCs and realize the dual effects of environmental remediation and power output.
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