In order to investigate the effect of different hydrothermal pretreatment intensities on the struc-ture and composition of Miscanthus,especially to analyze and compare the effects of different pretreatment conditions on the enzymatic saccharification of Miscanthus.Different pretreatment methods such as hydro-thermal with acid or ethanol,deep eutectic solvents were compared.The effect of temperature,time,solid-liquid ratio,and acetic acid concentration on the composition of Miscanthus during the hydrothermal process were investigated.The results showed that the highest glucose yield reached 65.43％without the addition of acetic acid when pure hydrothermal pretreatment at 180 ℃,with a reaction time of 2.5 h and a solid-liquid ratio of 1∶7.Compared with other pretreatment methods,this process has the advantages of simplicity,low cost,and efficient utilization of biomass resources.
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