Combined plant-microbe treatment for oil-contaminated soil remediation
The present study was conducted to promote the remediation of oil-contaminated soils near the Zhidan oilfield using combined plant-microbe treatments.Two plants,ryegrass(63.28%)and alfalfa(59.31%),were selected for their significant remediation capabilities in petroleum-contaminated soil based on their seed tolerance to petroleum-contaminated soil,morpho-physiological indices,and their abil-ity to degrade soil petroleum hydrocarbons.A microbial fungicide,ZD,with efficient remediation capabili-ties for petroleum-contaminated soil,was isolated from the rhizosphere of ryegrass and alfalfa.The use of ryegrass and alfalfa in conjunction with the composite microbial fungicide ZD for the remediation of petro-leum-contaminated soil revealed that an inoculum of 1.25%of microbial fungicide ZD yielded a notable effectiveness(78.28%)in the combined plant-microbe remediation of petroleum-contaminated soil.This study successfully obtained plants and microbial agents from petroleum-contaminated soil,and after proper treatment,reused them for the efficient remediation of petroleum-contaminated soil,aligning with the prin-ciple of utilizing local resources for remediation purposes,as exemplified by the concept"what is taken from here,is used in here."