Synthesis,isolation and antioxidant activity of astaxanthin palmitate
Astaxanthin palmitate diester was synthesized by esterification of astaxanthin and palmitic acid,and the optimum reaction conditions were as follows:50 mg of astaxanthin,45 mg of palmitic acid,1 mL of dichloromethane,80 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 40 mg of 4-dimethyl-aminopyridine,under nitrogen protection at 25℃for 12 h.Astaxanthin palmitate diester and astaxanthin palmitate monoester were purified by column chromatography with purities of 98.9%and 97.4%,respec-tively.The structure of the product was characterized by IR,NMR and high-resolution mass spectrometry.The antioxidant experiments showed that the overall antioxidant effect was astaxanthin palmitate monoester>astaxanthin>astaxanthin palmitate diester.
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