烟草Nt-GAD的氨基酸序列分析
Amino Acid Aequence Analysis of Nt-GAD in Tobacco
龙岗 1陈树鸿 1龙建林 1高萍 1赵娟1
作者信息
- 1. 四川省烟草公司 凉山州公司会理分公司,四川 会理 615100
- 折叠
摘要
为从分子层面研究烟草Nt-GAD的氨基酸序列,采用Protparam、Interpro、Blast、Muscle、Phyml和Treedyn工具,分析烟草Nt-GAD氨基酸序列的理化特性、结构域和进化树.结果表明:烟草Nt-GAD成员的等电点均处于 5.5～6 之间,且Nt-GAD1 和Nt-GAD2 同源性最高,此外Nt-GAD2 的耐热性最好,Nt-GAD3 的稳定性最强;烟草Nt-GAD属于磷酸吡哆醛依赖性脱羧酶家族和谷氨酸脱羧酶家族,且具有磷酸吡哆醛依赖性转移酶同源超家族结构域,C末端存在独特区域钙调蛋白结构域,通过进化树分析烟草Nt-GAD成员被划分为三组,其中烟草Nt-GAD3(第一组)分化较为突出,而Nt-GAD2(第三组)则较为保守,而Nt-GAD1 和Nt-GAD4 被划分为第二组.
Abstract
To investigate the amino acid sequence of tobacco Nt-GAD at the molecular level,the physical and chemical characteristics,structural domains and evolutionary trees of tobacco Nt-GAD amino acid sequence were analyzed using the tools of Protparam,Interpro,Blast,Muscle,Phyml and Treedin.The results showed that the isoelectric points of tobacco Nt-GAD members ranged between 5.5～6,and Nt-GAD1 exhibited the highest homology with Nt-GAD2.In addition,Nt-GAD2 had the best heat resistance and Nt-GAD3 had the strongest stability.Tobacco Nt-GAD was classified within the pyridoxal phosphate-dependent decarboxylase fami-ly and the glutamate decarboxylase family,possessing the pyridoxal phosphate-dependent transferases homologous superfamily domain.A distinctive calmodulin domain was located at the C-terminal.Based on evolutionary tree analysis,tobacco Nt-GAD members were categorized into three groups.Among these group,tobacco Nt-GAD3(group 1)is more prominent,whereas Nt-GAD2(group 3)is more conservative,Nt-GAD1 and Nt-GAD4 con-stitute the second group.
关键词
烟草/谷氨酸脱羧酶/生物学Key words
Tobacco/Glutamate decarboxylase/Biology引用本文复制引用
出版年
2024
NETL
NSTL
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