Breeding of High-Produing Cellulase Strain and Their Enzyme Production Process
In order to obtain the strain with high cellulase yield,this study used Cellulomonasuda as the re-search objec,the strain mutation by ultraviolet mutagenesis,the selected medium of Congo red ellulase activity to obtain strain W3,with enzyme activity up to 37.6 U/mL.Compared with the initial strain,the increase was 49.4％.The high enzyme activity after ten generations,indicating that the mutant strain had stable genetic charac-ter and strong cellulase production ability.The single factor optimization experiment was to the optimal culture con-ditions of the mutant strain,and then the best parameters were determined by orthogonal experiment for the key factors.The optimal culture conditions were as follows:12.5 g/L sucrose,2 g/L ammonium nitrate,2 g/L potassium dihydrogen phosphate,3％inoculation volume ratio of bacterial suspension,pH=6.0,fermentation culture tem-perature 35℃.The optimized enzyme activity reached 43.06 U/mL,which was 78.7％higher than that of the orig-inal strain.The results can provide the oretical basis and application value for the efficient degradation of cellulose.
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