Study on Anti-browning in Tissue Culture of'Xiaoguo Sweet Persimmon'
To reduce browning in tissue culture of'Xiaoguo Sweet Persimmon',a study on tissue culture in anti-browning of'Xiaoguo Tianshi'was conducted using its tissue culture progeny as materials from three as-pects:the type of basic medium,the concentration of cytokinin and the concentration of anti-browning agent.The results showed that,compared with(1/2N)MS,(3/4N)MS,and MS basic medium,DKW medium exhibi-ted the lightest browing,which was confined to the base of plant and did not spread throughout the medium.The effectiveness of cytokinin ZT alone was better than that of 6-BA alone or ZT + 6-BA combination.Anti-browning agents AC and riboflavin demonstrated inhibitory effect on the browning of'Xiaoguo Sweet Persimmon'subculture seedlings,whereas PVP did not exhibited inhibitory effects on browning.AC showed superior efficacy compared to riboflavin and PVP;however,both AC and riboflavin could inhibit plant growth and affect subcul-ture proliferation.Therefore,the optimal conditions for proliferation and anti-browning effects of subcultured plantlets of'Xiaoguo Sweet Persimmon'were achieved when DKW was used as the basic medium,supplemented with ZT(2.0 mg/L)without the addition of any anti-browning agent.
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