Development and Application of Markers Related to Yellow-Heart Traits in Chinese Cabbage
At present,research on selecting the yellow-heart trait in Chinese cabbage is limited,and there is insufficient experimental evidence.This study is centered on molecular marker research with the objective of validating and screening the yellow-heart trait in Chinese cabbage.The aim of this study is to find the precise molecular markers for screening this trait,thus laying a foundation for further study of traits in yellow-heart Chi-nese cabbage,and meeting the increasing consumer demand for yellow-heart Chinese cabbage in the agricultural market.'127-4'(P1)and'11-2'(P2)were used as the main materials,and'127-4'as the male parent and'11-2'as the female parent,after the F1 generation was obtained from the cross,the self-cross of F1 generation was continued to obtain'264'(F2)population.Using the obtained Chinese cabbage plants,the whole genome of Chinese cabbage was re-sequenced by BSA-seq method,and the primers were designed and screened for i-dentification,finally,the tag was screened as"9-13"(F:5'-CCGAAGCCCAGATTAGGAG-3';R:5'-ccat-gcctacgaatgatat-3').The molecular marker could extend a specific band of 200 bp in the breeding materials of yellow-heart Chinese cabbage.The results showed that the coincidence of the marker between white-heart Chinese cabbage and yellow-heart Chinese cabbage was more than 75%.At present,more than 30 germplasm resources of Chinese cabbage have been accurately screened by using this marker,which has reduced the field work for growers,the traits of Chinese cabbage characters have been specially controlled,laying a foundation for the accuracy of subsequent screening.
Chinese cabbageYellow-heart traitMolecular markerBSA sequencingGermplasm screening
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