蝴蝶兰'大辣椒'花粉活力及柱头可授性探究
Pollen Viability and Stigma Receptivity of Phalaenopsis'Big Chili'
张果 1王世尧 1杨书才 1蒋拴丽 1王瑞华 1赵玉安 1冯建 1杨录军 1王俊1
作者信息
- 1. 郑州市农林科学研究所,河南 郑州 450005
- 折叠
摘要
以蝴蝶兰'大辣椒'的不同时期花朵为试验材料,利用TTC和联苯胺-过氧化氢法分别检测花粉活力和柱头的可授性,探索最佳的授粉时期,以期为杂交育种选择亲本提供参考依据.结果表明,随着开放时间的增长,蝴蝶兰花粉团颜色逐渐加深,体积变小,硬度增加;蝴蝶兰不同开放时期花粉最佳染色时间不同,花蕾期为24 h,其余时期为48 h;不同开放时期花粉的活力也有差异,小花蕾期>大花蕾期>开放1d>开放5～15d;小花蕾期柱头可授性较弱,大花蕾、开放1d和开放5d柱头可授性逐渐有所增强,开放10d和开放15d柱头可授性最强.
Abstract
In this paper,we utilized the different flowering stages of Phalaenopsis'Big Chili'as the experi-mental material to detecte the pollen viability by TTC staining method and determine the stigma receptivity by benzidine-hydrogen peroxide method.The objective was to explore the optimal pollination period and provide a reference for parent selection in hybrid breeding.The results showed that the color of Phalaenopsis pollen dark-ened,its volume decreased,and its texture hardened with advancing flower opening time.The optimal time for pollen staining of Phalaenopsis varied with flowering stages,being 24 h during the bud stage and 48 h during sub-sequent periods.There were also differences in pollen viability at different flowering stages,with the order of small bud stage>large bud stage>one day after opening>5 d-15 d after opening.Stigma receptivity was weak at small bud stage,enhanced at large bud,one day and five days after opening,and strongest at ten day and fifteen days after opening.
关键词
蝴蝶兰/花粉活力/柱头可授性Key words
Phalaenopsis/Pollen viability/Stigma receptivity引用本文复制引用
基金项目
河南省重点研发与推广专项(科技攻关计划)(2023)(232102111101)
河南省林木种质资源建设蝴蝶兰种质资源建设项目(豫财环资[2023]60号)
出版年
2024
NETL
NSTL
万方数据