Optimization of Fermentation Conditions for Salinomycin Production by Streptomyces albus Ys6-101
As a pharmaceutical feed additive,salinomycin has been widely used and popularized in the poul-try industry.However,due to the high fuel consumption and high cost of its main production source,the indus-trial production of salinomycin has been greatly hindered.In order to optimize the salinomycin fermentation process and improve the industrial yield of salinomycin,this study explored and optimized the cultivation condi-tions of salinomycin seeds by altering the post-fermentation pH and inoculum size of primary and secondary seed liquids based on the laboratory-selected strain-Streptomyces albicans Ys6-101.The results showed that the pH of 6.80 and the inoculum amount of 18%were the optimum pH and inoculum amount of Streptomyces albus at seed cultivation stage,respectively.Meanwhile,controlling the original pH for primary seed and secondary seed were at 6.80,and the inoculation amount for primary seed and secondary seed were at 20 mL/110 L and 18%,the titer of salinomycin in the fermentation broth increased by 12.5%.Therefore,choosing the appropriate inoc-ulum size and pH after elimination can not only maximize the efficiency of the fermenter,but also help to shorten the fermentation cycle,improve the fermentation titer,and reduce production cost.
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