摘要
为探讨冀北水稻田土壤细菌群落结构和多样性,以承德地区水稻主产区(河北隆化,河北滦平)稻田土壤为试验材料,采用高通量测序技术,对水稻大田土壤细菌群落结构和多样性进行研究.结果 表明:不同地区水稻田土壤微生物多样性存在差异,其中样本ZYY.CKD9 中微生物多样性最高且有益菌较多,主要有硫杆菌属(Thiobacillus)、拟衣藻属(Dechloromonas)和地杆菌属(Geobacter);但也存在共有细菌菌群,如Kaistobacter、溶杆菌属(Lysobacter)、乳酸杆菌属(Lactobacillus)、Candidatus_Solibacter、硫杆菌属(Thiobacillus)、红长命菌属(Rubrivivax)、拟衣藻属(Dechloromonas)、Anaeromyxobacter、硝化螺旋菌属(Ni-trospira)等;PCA分析结果显示,土壤细菌群落结构空间相关性不明显.这为冀北水稻田土壤修复、病害防治及功能菌肥开发,促进水稻高产栽培提供依据.
Abstract
To explore structure and diversity of soil bacterial communities in paddy fields in North Hebei,soil samples were collected from 10 main producing areas in Longhua and Luanping in Hebei.High-throughput se-quencing technology was used to investigate the community structure and diversity of soil bacteria in paddy fields.The results showed that ZYY.CKD9 had the highest microbial diversity and a large number of beneficial bacteri-a,including Thiobacillus,Dechromonas,and Geobacter;However,there were also common bacterial communi-ties such as Kaistobacter,Lysobacter,Lactobacillus,Candidatus_Solibacter,Thiobacillus,Rubrivivax,Dechlo-romonas,Anaeromyxobacter,Nitrospira;The PCA analysis results showed that the spatial correlation of soil bac-terial community structure was not significant.This study provides a basis for soil restoration,disease prevention and control,and the development of functional bacterial fertilizers to promote high-yield cultivation of rice in North Hebei.
基金项目
承德市科技局承德市基础研究项目(2022)(202205B064)
承德市农林科学院自主科研项目(202301A010)