The biological control efficacy against Myzus persicae(Sulzer)and Thrips tabaci(Lindeman)was evaluated by releasing Aphidius gifuensis(Ashmead)and Neoseiulus barkeri(Hughes)into a tobacco field in Miyi,Panzhihua,Sichuan Province,for two years.The effect on tobacco aphids,thrips,and other insects was assessed during the presence of the released natural enemy insects.The results showed that:the control effect of Aphidius gifuensis against Myzus persicae and Neoseiulus barkeri against Myzus persicae was 6.18%and 33.94%in the earlier stage,respectively,and increased to 55.38%and 65.00%later on.However,the effect of imidacloprid treatment was the opposite.Both Aphidius gifuensis and Neoseiulus barkeri had reduced the popu-lations of other insects in the field to 25.12%and 20.02%,respectively,which were far lower than the effect of imidacloprid(53.29%).The population of Syrphidae was most affected with reductions of 39.36%and 51.85%,followed by Coccinella with reductions of 20.77%and 1.57%,while spiders were minimally affected at 12.46%and 1.60%by the natural enemy insects.This study indicates that Aphidius gifuensis and Neoseiulus barkeri are eco-friendly and highly effective for the long-term control of tobacco aphids and thrips.Bio-control technologies like these could be applied in the future.