This study aimed to determine the effect of electron beam irradiation on the degradation of afla-toxins(AFB1,AFB2,AFG1,AFG2)and spinosin in Ziziphi Spinosae Semen.The effects of different irradiation doses and different water contents on the contents of aflatoxins(AFB1,AFB2,AFG1,AFG2)and spinosin in Ziziphi Spinosae Semen were investigated using high-performance liquid chromatography.The results showed that the degradation rates of AFB1,AFB2,AFG1,and AFG2 treated with acetonitrile solution increased with irra-diation dose,and the degradation rates of AFB1,AFB2,AFG1,and AFG2 treated with 8 kGy irradiation were 97.25%,93.59%,75.11%,and 69.70%,respectively.With the increase of irradiation dose and water con-tent,the degradation rate of aflatoxin in Ziziphi Spinosae Semen also increased.The aflatoxin degradation rates of Ziziphi Spinosae Semen with 23%water content were 30.58%,30.24%,38.04%,and 36.58%,respectively.There was no significant difference in the content of spinosin after different irradiation doses(P>0.05).The content of spinosin in Ziziphi Spinosae Semen irradiated with 7%water content was significantly higher than that in Ziziphi Spinosae Semen with water contents of 15%,19%,and 23%.In conclusion,electron beam irradia-tion combined with water treatment can effectively degrade aflatoxin in Ziziphi Spinosae Semen;however,too high water content is not conducive to the maintenance of spinosin content.