Cellular oxidative damage detection based on Raman-enhanced property of nanomaterials
Surface-enhanced Raman spectroscopy(SERS)can be used as an assay for single-cell oxidative damage.A Raman spectroscopic characterization method was developed to study cellular damage using copper ion-induced oxidative damage in cells as a model and surface-enhanced Raman spectroscopy as a detection method.Gold nanoflowers with peptide modifications were prepared as Raman-enhanced substrate to enable them to enter cells and achieve signal enhancement.The extent of cell damage caused by the substrate was tested using CCK-8 cytotoxicity assay,and concentration of the substrate was determined to be 160 μg/mL.The cell oxidative damage caused by Cu2+was also verified through CCK-8 cytotoxicity assay.and significant damage and death of cells were observed under the inducement of Cu2+.Finally,a model of Cu2+-induced cell oxidative damage was established and Raman spectra presented by cells under different conditions were obtained.It was found that cells treated with oxidation were damaged to different degrees,and intracellular glycans,nucleic acids,lipids and proteins were altered to some extent,causing damage to the organism.
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