Lycium ruthenicum,rich in anthocyanins,is a medicinal and edible plant with high development and utilization value.In this study,aseptic seedling leaves of Lycium ruthenicum were used as explants to induce callus formation and establish a cell suspension system.The impacts of different exogenous hormones and temperatures on the anthocyanin content in suspension cells were investigated.The optimal culture system for callus induction was WPM+0.5 mg/L KT+2.0 mg/L 2,4-D+20.0 g/L sucrose+6.0 g/L agar,and the optimal system for suspension cell culture was WPM+0.5 mg/L KT+2.0 mg/L 2,4-D+0.01 mg/L NAA+20.0 g/L sucrose.The growth curve exhibited an'S'-shaped pattern,with the number of viable cells peaking at 7.93 × 105 cells/mL after 18 days of suspension culture.The treatments with 30 g/L sucrose,200 mg/L methyl jasmonate,2 mg/L abscisic acid and 16 ℃ all can significantly enhance the production of anthocyanin in suspension cells(P＜0.05).Among these treatments,the induction effect of 2 mg/L abscisic acid was the best.For the combined treatments of 16 ℃ and 3 exogenous hormones,the combined induction treatment of 200 mg/L methyl jasmonate+16 ℃ could significantly enhance the anthocyanin accumulation in suspension cells(P＜0.05),resulting in 1.13 times higher anthocyanin content than that of the 16 ℃ treatment alone.The results laid a foundation for further optimizing the suspension cell culture system and promoting anthocyanin accumulation in Lycium ruthenicum.
维普
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