The expression characteristics of PwRhomboid gene in Picea wilsonii and its effects on drought resistance
By sequencing and analyzing the transcriptome of Picea wilsonii,the potential drought-related gene PwRhomboid was screened out,and its functional characteristics of drought resistance in Picea wilsonii were analyzed.Through bioinformatics analysis,the amino acid sequence and homologous gene of PwRhomboid were compared.The responses of PwRhomboid to different abiotic stresses and hormones were analyzed by RT-qPCR.Instantaneous transformation of tobacco leaves was conducted to detect the subcellular localization of PwRhomboid protein.PwRhomboid transgenic strains of Arabidopsis and potato were obtained and their drought phenotypes were verified.The results showed that the amino acid sequence of PwRhomboid has significant difference with those of its homologous species at N-terminus,but shows high similarity and good conservation at C-terminus.PwRhomboid protein is mainly localized on cell membrane.The expression level of PwRhomboid was significantly induced by drought,low temperature and abscisic acid,with the highest expression level observed in mature leaves.PwRhomboid can improve the drought resistance in transgenic Arabidopsis and potato.After drought treatment,compared with the control group Arabidopsis WT and pCM1205 strains,PwRhomboid overexpression strains PwRhomboid-L1 and PwRhomboid-L2 had higher survival rates,chlorophyll fluorescence Fv/Fm and ΦPSⅡ.After polyethyleneglycol simulated drought treatment,the overexpression strains PwRhomboid-L3 and PwRhomboid-L4 resulted in higher potato seedling heights compared to the wild-type.The results showed that the expression level of PwRhomboid is affected by drought and hormones,and overexpression of PwRhomboid could improve the drought resistance of Arabidopsis and potato.
万方数据
维普
