Overexpression of AetHIPP28 enhances cadmium tolerance in Arabidopsis thaliana
Aegilops tauschii,the D genome donor of wheat(Triticum aestivum),has a variety of genes resistant to biotic and abiotic stresses,and is the main gene pool for wheat genetic improvement.Heavy metal-associated isoprene plant protein(HIPP)plays an important role in regulating metal ion balance and detoxification in plants.In this study,the transgenic Arabidopsis thaliana overexpressing AetHIPP28 was obtained by Agrobacterium-mediated method,and the response of transgenic Arabidopsis thaliana to cadmium(Cd)stress was studied by a series of physiological and biochemical analyses.The results showed that AetHIPP28 was localized in the nucleus and plasma membrane.Overexpression of AetHIPP28 significantly enhanced Arabidopsis thaliana tolerance to Cd,and promoted the root growth of Arabidopsis thaliana under Cd stress.The ROS accumulation of transgenic Arabidopsis thaliana under Cd stress was significantly lower than that of the wild type,while the activity of antioxidant enzymes was significantly higher than that of the wild type.Meanwhile,overexpressing AetHIPP28 inhibited Cd2 influx and increased Cd accumulation in roots.These results suggest that overexpression of AetHIPP28 improves Cd tolerance in Arabidopsis thaliana by regulating ROS accumulation,antioxidant enzyme activity,Cd2+influx and distribution in roots,which provide a reference for investigating the response mechanism of AetHIPP28 to Cd stress.
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