Cloning and expression analysis of VvAGL12 gene promoter in Vitis vinifera
The MADS-box family is one of the largest transcription factor families in plants,and plays an important role in adversity stress and flowering response.The function of AGL12-like subgroup in Vitis vinifera remains unclear.The VvAGL12 promoter(proVvAGL12)was cloned from the Pinot Noir grape genome,and the sequence elements of the promoter were analyzed.It was found that there were several cis-acting elements related to adversity stress in the promoter region.GUS expression vector driven by proVvAGL12 was constructed and transformed in Arabidopsis thaliana and tobacco.It was found that proVvAGL12 promoter fragment had activation activity in Arabidopsis thaliana,and it can drive GUS expression in leaves,stem segments,flower organs,roots and fruit pods in transgenic Arabidopsis thaliana plants.The expression activity can last the whole growth cycle.Transgenic Arabidopsis thaliana and tobacco stress treatment showed that the activity of proVvAGL12-driven GUS was regulated by gibberellin,abscisic acid,polyethylene glycol and low temperature.
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